While isolating DNA from bacteria, which of the following enzymes is not used ?

Options

A. a) Lysozyme
B. b) Ribonuclease
C. c) Deoxyribonuclease
D. d) Protease

Explanation

To solve the question regarding which enzyme is not used while isolating DNA from bacteria, we will analyze the functions of the given enzymes step by step.

### Step-by-Step Solution:

1. **Understanding the Process of DNA Isolation**:
- DNA isolation involves breaking open the bacterial cells to release DNA. This process typically requires several enzymes to break down different components of the cell.

2. **Analyzing Each Enzyme**:
- **Option A: Lysozyme**:
- Function: Lysozyme breaks down peptidoglycan in the bacterial cell wall.
- Importance: It is crucial for lysing the bacterial cells to release DNA. Therefore, it is required for DNA isolation.

- **Option B: Ribonuclease (RNase)**:
- Function: Ribonuclease digests RNA.
- Importance: It is important to remove RNA from the sample to ensure that only DNA remains. Thus, it is also required for DNA isolation.

- **Option C: Deoxyribonuclease (DNase)**:
- Function: Deoxyribonuclease cleaves DNA.
- Importance: This enzyme would degrade the DNA, which is counterproductive to the goal of isolating DNA. Therefore, it is not required for the isolation process.

- **Option D: Protease**:
- Function: Protease breaks down proteins.
- Importance: It is necessary to remove proteins from the sample, allowing for the purification of DNA. Hence, it is required for DNA isolation.

3. **Conclusion**:
- After analyzing all the options, the enzyme that is not used in the process of isolating DNA from bacteria is **Option C: Deoxyribonuclease**.

### Final Answer:
The enzyme that is not used while isolating DNA from bacteria is **C. Deoxyribonuclease**.

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