Which of the following should be chosen for best yield if one were to produce a recmobinant protein in large amounts ?
(a) Laboratory flask of largest capacity
(b) A stirred-tank bioreactor without in-lets and out-lets
(c) A continuous culture system
(d) Any of the above

Options

A. Laboratory flask of largest capcity
B. A stirred-tank bioreactro without in-lets and out-lets
C. A continuous culture system
D. Any of the above

Explanation

To determine the best option for producing a recombinant protein in large amounts, we need to analyze each of the given choices:

1. **Laboratory flask of largest capacity (Option A)**:
- A laboratory flask, regardless of its capacity, is not designed for large-scale production. It is typically used for small-scale experiments and does not provide the necessary conditions for optimal growth and protein production. The cells will eventually reach a stationary phase, limiting the yield of recombinant protein.

2. **A stirred-tank bioreactor without inlets and outlets (Option B)**:
- A stirred-tank bioreactor is generally suitable for large-scale production, but if it lacks inlets and outlets, it cannot efficiently manage the supply of fresh nutrients or the removal of waste products. This would lead to suboptimal growth conditions and reduced protein yield, as the cells would not be able to thrive in a stagnant environment.

3. **A continuous culture system (Option C)**:
- A continuous culture system allows for the continuous addition of fresh medium and the removal of spent medium. This setup keeps the cells in the exponential growth phase for a longer period, maximizing biomass and, consequently, the yield of recombinant protein. This method is ideal for large-scale production as it maintains optimal growth conditions.

4. **Any of the above (Option D)**:
- Since we have established that options A and B are not suitable for large-scale production, this option cannot be correct.

**Conclusion**:
The best choice for producing a recombinant protein in large amounts is **Option C: A continuous culture system**.