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Assertion : The matrix used in gel elect...

Assertion : The matrix used in gel electrophoresis should have controllable pore size.
Reason : Agarose concentration can be changed to change pore sizes.

A

a) Both assertion and reason are true and reason is correct explanation for assertion

B

b) Both assertion and reason are true but reason is not correct explanation of assertion

C

c) Assertion is true but reason is false.

D

d) Both assertion and reason are false.

Text Solution

AI Generated Solution

The correct Answer is:
**Step-by-Step Text Solution:** 1. **Understanding the Assertion**: The assertion states that the matrix used in gel electrophoresis should have controllable pore size. This is indeed true because the pore size of the gel matrix affects the movement of DNA molecules during electrophoresis. If the pore size is too large, DNA may leak out, and if it is too small, it can hinder the movement of DNA, making separation difficult. 2. **Understanding the Reason**: The reason provided states that agarose concentration can be changed to change pore sizes. This is also true. By adjusting the concentration of agarose in the gel, one can manipulate the size of the pores. Generally, a higher concentration of agarose results in smaller pore sizes, while a lower concentration leads to larger pores. 3. **Evaluating the Relationship**: While both the assertion and reason are true, the reason does not serve as a correct explanation for the assertion. The assertion is focused on the necessity of controllable pore sizes for effective DNA separation, while the reason merely states a method (changing agarose concentration) to achieve varying pore sizes. 4. **Choosing the Correct Option**: Based on the evaluation, the correct choice is that both the assertion and reason are true, but the reason is not the correct explanation for the assertion. Thus, the answer is: **Both assertion and reason are true, but reason is not the correct explanation for assertion.** ---
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