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Assertion : E.coli having pBR322 with DN...

Assertion : E.coli having pBR322 with DNA insert at BamHI site cannot grow in medium containing tetracyline.
Reason : Recoginition site for BamHI is present in `ter^(R)` region of pBR22.

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The correct Answer is:
To solve the question, we need to analyze both the assertion and the reason provided. ### Step-by-Step Solution: 1. **Understanding the Assertion**: - The assertion states that E. coli containing the plasmid pBR322 with a DNA insert at the BamHI site cannot grow in a medium containing tetracycline. - This is true because the presence of a DNA insert at the BamHI site disrupts the tetracycline resistance gene (ter^R) present in pBR322. When this gene is disrupted, E. coli loses its ability to resist tetracycline, leading to its inability to grow in a tetracycline-containing medium. 2. **Understanding the Reason**: - The reason states that the recognition site for BamHI is present in the tetracycline resistance region of pBR322. - This is also true. The BamHI restriction enzyme recognizes a specific sequence that is located within the tetracycline resistance gene of the pBR322 plasmid. 3. **Connecting Assertion and Reason**: - Since the reason correctly explains why the assertion is true (the insertion at the BamHI site inactivates the tetracycline resistance), we conclude that both the assertion and the reason are true, and the reason is the correct explanation for the assertion. 4. **Final Conclusion**: - Based on the analysis, the correct option is that both the assertion and the reason are true, and the reason is the correct explanation for the assertion. ### Final Answer: - Both assertion and reason are true, and the reason is the correct explanation for the assertion.
To solve the question, we need to analyze both the assertion and the reason provided. ### Step-by-Step Solution: 1. **Understanding the Assertion**: - The assertion states that E. coli containing the plasmid pBR322 with a DNA insert at the BamHI site cannot grow in a medium containing tetracycline. - This is true because the presence of a DNA insert at the BamHI site disrupts the tetracycline resistance gene (ter^R) present in pBR322. When this gene is disrupted, E. coli loses its ability to resist tetracycline, leading to its inability to grow in a tetracycline-containing medium. ...
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