Some of the step involved in the production of humulin are given below. Arrange them in the correct sequence and select the correct option
(i) Synthesis of gene (DNA) for human insulin artifically
(ii) Culturing recombinant E.coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into plasmid
(vi) Etraction of recombinant gene product from E.coli.

Options

A. (ii),(i),(iv),(iii),(v),(vi)
B. (i),(iii),(v),(vi),(ii),(iv)
C. (i),(iv),(v),(ii),(vi),(iii)
D. (iii),(v),(ii),(i),(vi),(iv)

Answer

To arrange the steps involved in the production of Humulin (human insulin) in the correct sequence, we need to follow the logical order of the biotechnological process. Here’s the step-by-step solution:

1. **Synthesis of gene (DNA) for human insulin artificially**: The first step involves creating the gene that encodes for human insulin. This is done through various techniques such as chemical synthesis or using recombinant DNA technology.

2. **Insertion of human insulin gene into plasmid**: After synthesizing the insulin gene, the next step is to insert this gene into a plasmid vector. This plasmid will carry the insulin gene into the host organism, which is typically E. coli.

3. **Culturing recombinant E. coli in bioreactors**: Once the plasmid containing the insulin gene is introduced into E. coli, the bacteria are cultured in bioreactors. This allows for the growth and multiplication of the bacteria, which will produce insulin.

4. **Extraction of recombinant gene product from E. coli**: After culturing, the next step is to extract the insulin produced by the recombinant E. coli. This involves breaking open the cells and isolating the insulin protein.

5. **Purification of Humulin**: The final step is to purify the extracted insulin to ensure it is suitable for medical use. This purification process removes any contaminants and ensures that the insulin is safe for injection into patients.

Thus, the correct sequence of steps is:
(i) → (iv) → (ii) → (vi) → (iii).