Which of the following statements is not correct ?

Options

A. Insulin used for diabetic patients was earlier extracted from pancreas of slaughtered cattle and pigs which was more efficient than the genetically engineered insulin
B. PCR technique is applied to detect HIV suspected AIDS patients and to detect mutations in genes in suspected cancer patients
C. Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes in ADA deficient patients
D. Bioremediation is the one of the applications of biotechnology.

Explanation

To determine which of the statements is not correct, we will analyze each statement one by one.

### Step-by-Step Solution:

1. **Statement 1**: "Insulin used for diabetic patients is extracted from the pancreas of slaughtered cattle and pigs which was more efficient than genetically engineered insulin."
- This statement is **not correct**. While it is true that insulin was historically extracted from the pancreas of cattle and pigs, this insulin was not more efficient than genetically engineered insulin. In fact, genetically engineered insulin is designed to be identical to human insulin, making it more effective and reducing the risk of allergic reactions.

2. **Statement 2**: "PCR is applied to detect HIV in suspected AIDS patients and to detect mutations in genes in suspected cancer patients."
- This statement is **correct**. Polymerase Chain Reaction (PCR) is a technique used to amplify DNA, allowing for the detection of viruses like HIV and genetic mutations associated with cancer, even in the absence of symptoms.

3. **Statement 3**: "Bone marrow transplantation requires periodic infusion of genetically engineered lymphocytes in ADA deficient patients."
- This statement is **correct**. Bone marrow transplantation can provide a temporary solution for ADA deficiency, but patients often require periodic infusions of genetically engineered lymphocytes to maintain their health.

4. **Statement 4**: "Bioremediation is one of the applications of biotechnology."
- This statement is **correct**. Bioremediation involves using living organisms, such as microbes, to remove contaminants from the environment, making it a recognized application of biotechnology.

### Conclusion:
The statement that is **not correct** is **Statement 1**.