Technique used to detect the DNA in a clone is

Options

A. polymerase chain reaction
B. gel electrophoresis
C. chromatography
D. autoradiography

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks for the technique used to detect DNA in a clone. This implies that we need a method that can identify and analyze specific DNA sequences.

2. **Evaluating the Techniques**:
- **Polymerase Chain Reaction (PCR)**: This technique is primarily used for amplifying DNA. It allows us to make multiple copies of a specific DNA segment, which can then be analyzed. This amplification is crucial for detecting DNA, especially when the sample is limited.
- **Gel Electrophoresis**: This method is used to separate DNA fragments based on size, but it does not directly detect DNA in a clone. It is often used after PCR to visualize the amplified DNA.
- **Chromatography**: This technique is generally used for separating mixtures and is not suitable for DNA detection.
- **Autoradiography**: This technique involves using X-ray film to visualize radioactive materials, but it is not applicable for detecting DNA in clones.

3. **Conclusion**: Among the techniques evaluated, PCR stands out as the most suitable method for detecting DNA in a clone because it amplifies the DNA, allowing for identification and analysis of specific sequences.

4. **Final Answer**: The technique used to detect the DNA in a clone is **Polymerase Chain Reaction (PCR)**.

---