Read the following statements regarding ELISA and select the incorrect one

Options

A. It is used for the early diagnosis of diseases
B. It is based on the principle of antigen-antibody interaction
C. Infection by pathogen can be detected the presence of antigenes like proteins and glycoproteins
D. none of these

Answer

To determine the incorrect statement regarding ELISA (Enzyme-Linked Immunosorbent Assay), we can analyze the provided information step by step.

### Step 1: Understand the Definition of ELISA
ELISA stands for Enzyme-Linked Immunosorbent Assay. It is a biochemical technique used to detect the presence of an antibody or an antigen in a sample.

**Hint:** Remember the full form of ELISA and its primary purpose.

### Step 2: Principle of ELISA
The method is based on the principle of antigen-antibody interaction. When an antigen enters the body, antibodies are produced to bind to it. This interaction is crucial for the functioning of ELISA.

**Hint:** Focus on the role of antigen-antibody interaction in ELISA.

### Step 3: Application of ELISA
ELISA is used for the early detection of various diseases by identifying the presence of specific antigens or antibodies in blood or other bodily fluids. It is particularly useful in diagnosing infections.

**Hint:** Think about how ELISA is applied in medical diagnostics.

### Step 4: Clarification of DNA Amplification
The video mentions DNA amplification and polymerase chain reaction (PCR) in relation to ELISA. However, it is important to note that PCR is a separate technique used for amplifying DNA, while ELISA is focused on detecting proteins or antibodies, not amplifying DNA.

**Hint:** Distinguish between ELISA and PCR; they serve different purposes.

### Step 5: Evaluate the Statements
Based on the understanding of ELISA:
1. It helps in early diagnosis - True.
2. It is based on the principle of antigen-antibody reaction - True.
3. It detects the presence of antigens like proteins and glycoproteins in the blood - True.
4. ELISA involves DNA amplification using PCR - This is incorrect as ELISA does not involve DNA amplification.

**Hint:** Identify which statement does not align with the core function of ELISA.

### Conclusion
The incorrect statement regarding ELISA is that it involves DNA amplification using PCR. ELISA is not a DNA amplification technique; it is used for detecting proteins or antibodies.

**Final Answer:** The incorrect statement is that ELISA involves DNA amplification using PCR.