Show with a sketch of E-coli cloning vector showing restriction sites.

List the features required to facilitate cloning into vector. Show with a sketch the E. coli cloning vector showing restriction site.

Define restriction site.

Write a note on cloning vector.

Name any one cloning vector.

What is the role of Ori for cloning vector ?

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?

explain pBR 322 plasmid and label the following in it: Any two restriction sites.

Redraw diagram of cloning vector pBR 322 given below and label the parts A, B , C, D showing restriction sites.

Show with graphic sketch the hormonal control over the menstrual cycle.