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The linking of antibiotic resistance gen...

The linking of antibiotic resistance gene with the plasmid vector became possible with :

A

DNA ligase

B

Exonucleases

C

Endonucleases

D

DNA polymerase

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The correct Answer is:
A
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Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

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The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

Explain antibiotic resistance observed in bacteria in light of Darwinian selection theory.

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In recombinant DNA technology, a plasmid vector is cleaved by

MBD PUBLICATION-PRINCIPLES AND PROCESS OF BIOTECHNOLOGY-QUESTION BANK
  1. The construction of the first recombinant DNA was done by using the na...

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  2. The basis of DNA fingerprinting is :

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  3. The linking of antibiotic resistance gene with the plasmid vector beca...

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  4. Gel electrophoresis is used for :

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  5. Which one of the following palindromic base sequences in DNA can be ea...

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  6. An improved variety of transgenic basmati rice :

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  7. Generic engineering has been successfully used for producing:

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  8. The genetically-modified (GM) brinjal in India has been developed for:

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  9. PCR and 'Restriction Fragment Length Polymorphism are the methods for ...

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  10. For transformation, microparticles coated with DNA are to be bombarded...

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  11. Which vector can clone only a small fragment of DNA ?

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  12. In vitro clonal propagation in plants is characterized by:

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  13. DNA fragments are :

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  14. What term is used for an extrachromosomal circular DNA in a bacterial ...

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  15. What are the molecular scissors ?

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  16. What term is used for the DNA sequence from same words when read both ...

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  17. Name the enzyme that joins two strands of DNA.

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  18. What type of technique is used for amplification of DNA ?

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  19. Name the protein that produced in a heterozygous host .

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  20. What is the first restriction endonuclease ?

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