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Gel electrophoresis is used for :...

Gel electrophoresis is used for :

A

isolation of DNA molecule

B

cutting of DNA into fragments

C

separation of DNA fragments according to their size

D

construction of recombinant DNA by joining with cloning vectors

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The correct Answer is:
C
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Gel electrophoresis is a

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

The anionic detergent, used in polyacrylamide gel electrophoresis is known as ……………………. .

Gel electrophoresis separates the DNA on the basis of its ---.

Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes.

The figure shows the restriction enzyme cutting sites (R1-R3) in wild type (n) and mutant (n) gene. If a radioactively labelled probe (that hybridises at a sequence close to R1) is used for detecting the presence of DNA fragments after gel electrophoresis and Southern blotting, which of the following band patterns will yout expect ? Note : L1 : wild type DNA, L2: mutant DNA

Explain electrophoresis.

MBD PUBLICATION-PRINCIPLES AND PROCESS OF BIOTECHNOLOGY-QUESTION BANK
  1. The basis of DNA fingerprinting is :

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  2. The linking of antibiotic resistance gene with the plasmid vector beca...

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  3. Gel electrophoresis is used for :

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  4. Which one of the following palindromic base sequences in DNA can be ea...

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  5. An improved variety of transgenic basmati rice :

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  6. Generic engineering has been successfully used for producing:

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  7. The genetically-modified (GM) brinjal in India has been developed for:

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  8. PCR and 'Restriction Fragment Length Polymorphism are the methods for ...

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  9. For transformation, microparticles coated with DNA are to be bombarded...

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  10. Which vector can clone only a small fragment of DNA ?

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  11. In vitro clonal propagation in plants is characterized by:

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  12. DNA fragments are :

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  13. What term is used for an extrachromosomal circular DNA in a bacterial ...

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  14. What are the molecular scissors ?

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  15. What term is used for the DNA sequence from same words when read both ...

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  16. Name the enzyme that joins two strands of DNA.

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  17. What type of technique is used for amplification of DNA ?

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  18. Name the protein that produced in a heterozygous host .

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  19. What is the first restriction endonuclease ?

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  20. Which acts as a vector to transfer a piece of DNA ?

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