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DNA fragments are :...

DNA fragments are :

A

Negatively charged

B

Nertral

C

Positively charged

D

Positively or negatively charged

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The correct Answer is:
A
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Match the terms given in column I with their definitions in column II and select the correct answer from codes given below. {:(Column I,Column II),(A."Transformation",(i)"Sequences cut by restriction enzymes"),(B."Recognition site",(ii)"Process by which DNA fragments are separated based on their size"),(C."Gel electrophoresis",(iii)"Plasmid DNA that has incorporated human DNA"),(D."Recombinant DNA",(iv)"Process by which bacteria take up pieces of DNA from the environment"):}

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

DNA fragments can be separated and detected using_____.

Fill up the blank DNA fragment are joined b y ____________

The figure shows the restriction enzyme cutting sites (R1-R3) in wild type (n) and mutant (n) gene. If a radioactively labelled probe (that hybridises at a sequence close to R1) is used for detecting the presence of DNA fragments after gel electrophoresis and Southern blotting, which of the following band patterns will yout expect ? Note : L1 : wild type DNA, L2: mutant DNA

Okazaki fragments are :

DNA fragments move towards anode during Denaturation.

MBD PUBLICATION-PRINCIPLES AND PROCESS OF BIOTECHNOLOGY-QUESTION BANK
  1. Which vector can clone only a small fragment of DNA ?

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  2. In vitro clonal propagation in plants is characterized by:

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  3. DNA fragments are :

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  4. What term is used for an extrachromosomal circular DNA in a bacterial ...

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  5. What are the molecular scissors ?

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  6. What term is used for the DNA sequence from same words when read both ...

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  7. Name the enzyme that joins two strands of DNA.

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  8. What type of technique is used for amplification of DNA ?

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  9. Name the protein that produced in a heterozygous host .

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  10. What is the first restriction endonuclease ?

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  11. Which acts as a vector to transfer a piece of DNA ?

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  12. Enzymes that cut the DNA at specific sites are called.

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  13. The enzyme required for making genes from the mRNA is called .

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  14. The plasmid of A. tumefacien is called .

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  15. Plants possessing genes of other organisms are called plants.

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  16. are most widely used cloning vectors in genetic engineering.

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  17. Short DNA molecules with known base sequences used for recognition of ...

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  18. As transgenic plants and animals produce warious useful substances, th...

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  19. Bt gene is transferred to plants from the bacterium

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  20. Seeds which cannot tolerate drying and freezing tempetaure are calleds...

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