Can you recall meiosis and indicate at what stage a recombinant DNA is made ?

What is a recombinant DNA?

What are the important steps in recombinant DNA technology ?

An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

Read the following statements and select the correct ones. (i) Electrophoresis is a technique used for the separation of molecules based on their size and charge. (ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeast,. (iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule. (iv) In EcoRl, the roman numberal I indicates that it was the first enzyme isolated from E.coli A) (i) and (ii) B) (iii) and (iv) C) (i),(ii) and (iv) D) (i),(ii),(iii) and (iv)

What are restriction endonucleases ? Mention their functions in recombinant DNA technology.