Can you thing and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker ?

Fill up the blanks with correct answer : DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called ____.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels

The DNA content of individual cells and the number of cells in each phase of a cell cycle" can be determined using flow cytometry. Which of the following combinations of "phase of a cell cycle and its corresponding DNA content" can be considered normal? (i) Diploid cells found in the G_(0) or G_(1) phase. (ii) Cells with twice the normal DNA content in the early M phase. (iii) Cells with twice the normal DNA content in the G_(2) phase.

Using recombinant DNA technology, genes from a donor cell can be inserted into a bacterium for DNA replication and protein synthesis. The kind of cells that can be used as gene donors in this technology are

Read the following statements and select the incorrect ones. (i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow. (ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules. (iii) Downstream processing is one of the steps of rDNA technology. (iv) Disarmed pathogen vectors are also used in transfer of rDNA into the host. a) (i) and (iii) b) (iii) and (iv) c) (i) and (iii) d) (i) and (ii)

An advantage of using yeasts rather than bacteria as recipient cells for the recombinant DNA of eukaryotes is that yeasts can

Do you think microbes can also be used as source of energy ? If yes, how ?

The given flow chart depicts the steps to transfer a desirable gene of interest into a plant. Identify the missing steps (A,B and C) with regard ot following statements and select the correct option. (i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule. (ii) Selection of transformed cells. (iii) Transferring the recombinant DNA molecules to teh target cells.

How can you prepare phenol by using benzene diazonium chloride ?