Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E.coli ?

A sequential expression of a set of human genes occurs when a steroid molecule bind to the

Read the given statements and select the correct option. Statement 1: Both bacteria and yeast multiply very fast to form huge populations which express the desired gene. Statement 2: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryotes).

The sequence of genes In lac operon in E. coli are :

Gene regulation governing lactose Operon E. Coli that involves the Lac i gene product is

Which gene controls transcription of chain A and chain B required for humalin synthesis in E.coli ?

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

List three different allelic forms of gene 'I' in human . Explain the different phenotypic expression, controlled by these three forms

If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grown and the untransformed recipient cells will die. The ampicillin resistant gene in this case is called as 1) selectable marker 2) recombinant protein 3) cloning site 4) chemical scalpels