Choose the correct match.

Options

A. Agrobacterium tumefaciens - Crown gall in dicot plants
B. Thermus aquaticus - Bt gene
C. Hind II - Plasmid vector
D. Ligase - Molecular scissors

Answer

To solve the question "Choose the correct match," we will analyze each option provided and determine if they correspond correctly with each other.

### Step-by-Step Solution:

1. **Analyze the first option: Agrobacterium tumefaciens and Crown Gall in Dicot plants.**
- Agrobacterium tumefaciens is a bacterium known to cause Crown Gall disease, which is characterized by tumor formation in dicot plants. This bacterium transfers a specific plasmid, known as the T.I. (Tumor Inducing) plasmid, into the plant cells, leading to the development of tumors.
- **Conclusion:** This match is correct.

2. **Analyze the second option: Thermus aquaticus and BT Gene.**
- Thermus aquaticus is a thermophilic bacterium from which Taq Polymerase is derived, used in PCR (Polymerase Chain Reaction). The BT Gene, on the other hand, refers to a gene from Bacillus thuringiensis, which is used for pest resistance in plants.
- **Conclusion:** This match is incorrect.

3. **Analyze the third option: Restriction enzymes and vector.**
- Restriction enzymes are proteins that cut DNA at specific sequences. A vector, however, is a vehicle used to transfer genetic material into a host cell. They are not the same; restriction enzymes do not serve as vectors.
- **Conclusion:** This match is incorrect.

4. **Analyze the fourth option: Ligase and scissors.**
- Ligase is an enzyme that joins DNA fragments together, while "scissors" typically refer to restriction enzymes, which cut DNA. Therefore, ligase does not match with scissors.
- **Conclusion:** This match is incorrect.

### Final Answer:
The only correct match is **Agrobacterium tumefaciens with Crown Gall in Dicot plants.**