Which of the following techniques is used for separation of DNA fragments during DNA fingerprinting?

Options

A. PCR
B. Autoradiography
C. Electrophoresis
D. Microarray

Answer

To solve the question regarding the technique used for the separation of DNA fragments during DNA fingerprinting, we will analyze the options provided in the context of their functions.

### Step-by-Step Solution:

1. **Understand the Techniques**:
- The question lists several techniques: PCR (Polymerase Chain Reaction), Autoradiography, Electrophoresis, and Microarray. We need to identify which of these is specifically used for separating DNA fragments.

2. **Evaluate PCR**:
- PCR is primarily used for amplifying DNA, meaning it increases the number of copies of a specific DNA segment. It does not separate DNA fragments; rather, it prepares them for analysis.

3. **Evaluate Autoradiography**:
- Autoradiography is a technique that uses X-rays to create images of radioactive materials. While it can visualize DNA, it does not separate DNA fragments.

4. **Evaluate Electrophoresis**:
- Electrophoresis, specifically Gel Electrophoresis, is a method that separates DNA fragments based on their size and charge. DNA fragments are placed in a gel, and an electric current is applied, causing the fragments to migrate through the gel. Smaller fragments move faster and farther than larger ones, effectively separating them.

5. **Evaluate Microarray**:
- Microarray technology is used for analyzing gene expression and can detect thousands of genes simultaneously. It does not separate DNA fragments but rather assesses their expression levels.

6. **Conclusion**:
- Based on the evaluations, the technique specifically used for the separation of DNA fragments during DNA fingerprinting is **Electrophoresis**.

### Final Answer:
**Electrophoresis** is the technique used for separation of DNA fragments during DNA fingerprinting.