Choose incorrect match amongst restriction enzymes listed in column l and type of ends produced in column ll

Options

A. Column I - EcoR I and Column II - Sticky end
B. Column I - Hind III and Column II - Sticky end
C. Column I - Sma I and Column II - Blunt end
D. Column I - Bam HI and Column II - Flush end

Answer

To solve the question, we need to identify the incorrect match between the restriction enzymes listed in column I and the type of ends produced in column II. Let's analyze each option step by step.

### Step-by-Step Solution:

1. **Understand the Types of Ends**:
- There are two main types of ends produced by restriction enzymes:
- **Sticky Ends**: These are irregular ends that have overhangs, allowing them to easily bind with complementary DNA fragments.
- **Blunt Ends**: These are straight cuts with no overhangs, resulting in flush ends.

2. **Analyze Each Enzyme**:
- **EcoRI**: This enzyme is known to produce sticky ends.
- **Match**: EcoRI - Sticky End (Correct)

- **HindIII**: This enzyme also produces sticky ends.
- **Match**: HindIII - Sticky End (Correct)

- **SmaI**: This enzyme produces blunt ends.
- **Match**: SmaI - Blunt End (Correct)

- **BamHI**: This enzyme is known to produce sticky ends, not blunt ends.
- **Match**: BamHI - Flush End (Incorrect)

3. **Conclusion**:
- The incorrect match among the options is **BamHI with Flush End**. Instead, BamHI produces sticky ends.

### Final Answer:
The incorrect match is **BamHI - Flush End**.

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