Which of the following restriction enzyme site is present in nucleotide sequence of ampicillin resistance gene in pBR322?

Options

A. Pvu I
B. Pvu II
C. Hind III
D. Sal I

Answer

To determine which restriction enzyme site is present in the nucleotide sequence of the ampicillin resistance gene in pBR322, we can follow these steps:

### Step-by-Step Solution:

1. **Understand the Context**: pBR322 is a well-known plasmid used as a cloning vector in molecular biology. It contains genes that confer resistance to antibiotics, specifically ampicillin and tetracycline.

2. **Identify the Genes**: The plasmid pBR322 has two antibiotic resistance genes:
- The **ampicillin resistance gene** (often referred to as **bla**).
- The **tetracycline resistance gene** (often referred to as **tet**).

3. **Know the Restriction Enzymes**: Familiarize yourself with the restriction enzymes that are commonly associated with pBR322:
- **PVU1**
- **PST1**
- **HIND3**
- **Sal-1**

4. **Match Restriction Enzymes to Genes**:
- PVU1 and PST1 are associated with the ampicillin resistance gene.
- HIND3 is found at the beginning of the plasmid.
- Sal-1 is associated with the tetracycline resistance gene.

5. **Select the Correct Option**: Since the question specifically asks for the restriction enzyme site present in the nucleotide sequence of the ampicillin resistance gene, the correct answer is **PVU1**.

6. **Final Answer**: The restriction enzyme site present in the nucleotide sequence of the ampicillin resistance gene in pBR322 is **PVU1**.