Eli Lilly an American company prepared two DNA sequences corresponding to A and B chains of human insuline and introduced them in

Options

A. E. Coli
B. B. thuringiensis
C. M. icognitia
D. Pseudomonas putida

Answer

**Step-by-Step Solution:**

1. **Understanding the Question**: The question asks about the introduction of two DNA sequences corresponding to the A and B chains of human insulin by the American company Eli Lilly.

2. **Background Information**: Eli Lilly is a well-known pharmaceutical company that has been involved in the production of various medications, including insulin. Insulin is a hormone crucial for regulating blood sugar levels in the body.

3. **DNA Sequences**: The A and B chains of human insulin are specific sequences of DNA that code for the production of the insulin hormone. These sequences are essential for the synthesis of functional insulin.

4. **Introduction into a Host**: To produce insulin, the DNA sequences of the A and B chains need to be introduced into a suitable host organism. In this case, the DNA sequences are introduced into a plasmid.

5. **Use of E. coli**: The plasmid containing the DNA sequences is then introduced into *Escherichia coli* (E. coli), a common bacterium used in genetic engineering. E. coli serves as a host for the production of insulin.

6. **Insulin Production**: Once the plasmid with the A and B chain sequences is inside the E. coli, the bacteria can use this genetic information to produce the insulin chains. The bacteria effectively become factories for insulin production.

7. **Extraction of Insulin**: After the E. coli has produced the insulin chains, these chains are extracted and combined to form human insulin, which can then be purified and used for medical purposes.

8. **Final Answer**: The two DNA sequences corresponding to the A and B chains of human insulin were introduced into the plasmid of *E. coli*.

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