The bacteria which do not retain the crystal violet of iodine stain when after washing with alcohol are included in

Options

A. Gram negative
B. Gram neutral
C. Grame positive
D. AF +ve

Answer

To solve the question, we need to understand the process of gram staining and the characteristics of different types of bacteria. Here’s a step-by-step solution:

### Step 1: Understand the Gram Staining Process
The question refers to bacteria that do not retain the crystal violet stain after washing with alcohol. This indicates that we are dealing with the gram staining process, which differentiates bacteria based on their cell wall structure.

**Hint:** Recall the steps involved in gram staining and the significance of crystal violet and alcohol in this process.

### Step 2: Differentiate Between Gram Positive and Gram Negative Bacteria
- **Gram Positive Bacteria:** These bacteria have a thick peptidoglycan layer in their cell wall. They retain the crystal violet stain even after the alcohol wash due to this thick layer.
- **Gram Negative Bacteria:** These bacteria have a thin peptidoglycan layer and an outer lipid membrane. They do not retain the crystal violet stain after washing with alcohol, which is why they lose the violet color.

**Hint:** Think about the structural differences in the cell walls of gram positive and gram negative bacteria.

### Step 3: Analyze the Question
The question specifically asks for bacteria that do not retain the crystal violet stain after washing with alcohol. This directly points to gram negative bacteria, as they lose the stain due to their thin peptidoglycan layer.

**Hint:** Focus on the key phrase "do not retain the crystal violet" to identify the correct category of bacteria.

### Step 4: Eliminate Incorrect Options
- **Gram Neutral:** These bacteria do not take up any stain, making them irrelevant to the question.
- **Gram Positive:** These bacteria retain the crystal violet stain, so they cannot be the answer.
- **AF Positive:** This refers to acid-fast bacteria, which is a different staining process altogether and not related to gram staining.

**Hint:** Consider the definitions of each option to rule out the incorrect answers.

### Conclusion
Based on the analysis, the correct answer to the question is **gram negative bacteria**.

**Final Answer:** Gram Negative