For its action, nitrogenase requires

Options

A. `Mn^(2+)`
B. Super oxygen radicals
C. High input of energy
D. Light

Answer

**Step-by-Step Solution:**

1. **Understanding Nitrogenase**: Nitrogenase is an enzyme involved in the process of nitrogen fixation, which is the conversion of atmospheric nitrogen (N₂) into ammonia (NH₃). This process is crucial for plants as they cannot utilize atmospheric nitrogen directly.

2. **Location of Nitrogenase**: This enzyme is primarily found in the root nodules of leguminous plants (e.g., peas, beans). These nodules are formed in symbiosis with nitrogen-fixing bacteria, such as Rhizobium.

3. **Energy Requirement**: For its action, nitrogenase requires a significant amount of energy. This energy is essential for the reduction of nitrogen gas to ammonia, a process that is energetically demanding.

4. **Co-factors and Conditions**: In addition to energy, nitrogenase also requires specific cofactors. These include magnesium ions (Mg²⁺) and other components that facilitate the enzymatic reaction. The presence of superoxide radicals may also play a role in the enzymatic activity.

5. **Overall Reaction**: The general reaction catalyzed by nitrogenase involves the conversion of nitrogen gas and hydrogen ions into ammonia, with the consumption of ATP (adenosine triphosphate) as the energy source.

6. **Conclusion**: Therefore, nitrogenase requires a high input of energy, magnesium ions, and possibly superoxide radicals for its action in nitrogen fixation.

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