You are given a tissue with its potential for differentiation in an artificial culture .Which of the following pairs of hormones would you add to the medium to secure shoots as well as roots

Options

A. IAA and gibberellin
B. Auxin and cytokinin
C. Auxin and abscisic acid
D. Gibberellin and abscisic acid

Answer

To solve the question regarding which pairs of hormones to add to the medium to secure both shoots and roots in a tissue culture, follow these steps:

### Step-by-Step Solution:

1. **Understand the Role of Hormones**:
- In plant tissue culture, hormones play a crucial role in differentiation. The two main types of hormones involved in shoot and root formation are auxins and cytokinins.

2. **Identify the Functions of Auxins and Cytokinins**:
- **Auxins**: These hormones are primarily responsible for root formation. They promote the development of roots when present in higher concentrations.
- **Cytokinins**: These hormones promote shoot formation. They encourage the growth of shoot buds when present in higher concentrations.

3. **Determine the Ratio for Differentiation**:
- The ratio of auxins to cytokinins is critical.
- If the ratio is equal, it typically results in the formation of an undifferentiated callus.
- A higher concentration of cytokinins leads to shoot formation, while a higher concentration of auxins leads to root formation.

4. **Conclusion**:
- To secure both shoots and roots, you need to add both auxins and cytokinins to the medium. The correct pairing of these hormones will allow for the differentiation of the tissue into both shoots and roots.

### Final Answer:
The correct pair of hormones to add to the medium is **Auxin and Cytokinin**.

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