For producing protoplasts form plant cells which of the following are required ?

Options

A. amylase and pectinase
B. cellulase and proteinase
C. cellulase and pectinase
D. cellulase and amylase

Answer

To produce protoplasts from plant cells, the following steps and requirements are necessary:

### Step-by-Step Solution:

1. **Understanding Protoplasts**: Protoplasts are plant cells that have had their cell wall removed, leaving only the plasma membrane and the cellular contents.

2. **Identifying Cell Wall Composition**: The plant cell wall is primarily composed of polysaccharides, mainly cellulose and pectin.

3. **Choosing the Right Enzymes**: To remove the cell wall, specific enzymes are required. The two key enzymes that are used for this purpose are:
- **Pectinase**: This enzyme breaks down pectin, a component of the plant cell wall.
- **Cellulase**: This enzyme breaks down cellulose, another major component of the plant cell wall.

4. **Combining the Enzymes**: A combination of pectinase and cellulase is used to effectively digest the cell wall and produce protoplasts.

5. **Conclusion**: Therefore, to produce protoplasts from plant cells, the required substances are pectinase and cellulase.

### Final Answer:
The correct answer is the combination of **pectinase and cellulase**.

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