In order to obtain disease free plants through tissue culture techniques the best method is

Options

A. embryo
B. anther cuture
C. protoplast culture
D. meristem culture

Answer

**Step-by-Step Text Solution:**

1. **Understanding the Question**: The question asks for the best tissue culture technique to obtain disease-free plants.

2. **Identifying Pathogens**: Plants can be infected by various pathogens, including viruses (which can be DNA or RNA-based) and bacteria. These pathogens can cause diseases in plants.

3. **Recognizing Meristematic Tissue**: In plants, there are specific regions known as meristems (such as shoot apical meristems and root apical meristems) that are responsible for growth and development. These regions are characterized by high metabolic activity.

4. **Explaining the Concept of Disease-Free Plants**: When plants are infected, pathogens thrive in stable conditions. However, meristematic tissues have a high rate of active metabolic activity, which creates an unstable environment for pathogens.

5. **Applying the Technique**: To obtain disease-free plants, we can take meristematic tissues from diseased plants and culture them. Since these tissues are less likely to harbor pathogens, they can be multiplied to produce healthy, disease-free plants.

6. **Conclusion**: The best tissue culture technique for obtaining disease-free plants is **meristem culture**.

---