The best way to obtain bacteria and viruses free plants through tissue culture is

Options

A. microporpaagation
B. seed germinatinn after gamma irradiation
C. stem or shoot tip culture
D. seed germination under aseptic condition

Answer

### Step-by-Step Solution:

1. **Understanding Tissue Culture**: Tissue culture is a technique used to grow plant cells, tissues, or organs in a controlled, sterile environment. It allows for the propagation of plants from small pieces of tissue, known as explants.

2. **Identifying Explants**: Explants can be taken from various parts of the plant, such as stems, leaves, or flowers. These explants are placed in a nutrient medium to encourage growth.

3. **Micropropagation**: While tissue culture is often referred to as micropropagation, it does not inherently guarantee that the plants produced will be free from bacteria and viruses.

4. **Gamma Radiation**: Seed germination after gamma radiation is a method that induces mutations but does not specifically target the elimination of viruses or bacteria. Therefore, it is not a suitable method for obtaining bacteria and virus-free plants.

5. **Stem or Shoot Tip Culture**: The most effective method for obtaining bacteria and virus-free plants through tissue culture is the use of stem or shoot tip culture. The meristematic cells located at the tips of stems or shoots are actively dividing and are typically free from viruses and bacteria.

6. **Conclusion**: Therefore, the best way to obtain bacteria and virus-free plants through tissue culture is to use stem or shoot tip culture.

### Final Answer:
The best way to obtain bacteria and virus-free plants through tissue culture is **stem or shoot tip culture**.

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