Sterilization of tissue culture medium is done by

Options

A. autoclaving of medium at `120^(@)C` for 15 minutes
B. filtering the medium through fine sieve
C. mixing the medium with afntifunagal
D. agents

Answer

### Step-by-Step Solution:

1. **Understanding the Need for Sterilization**:
- The primary reason for sterilizing tissue culture medium is to eliminate any potential contaminants, such as bacteria and fungi, that could interfere with the growth of the desired plant tissues.

2. **Identifying the Sterilization Method**:
- The most common method used for sterilizing tissue culture media is autoclaving. This process involves using a specialized device called an autoclave.

3. **Setting the Conditions for Autoclaving**:
- The autoclave operates by raising the temperature of the medium to approximately 120 degrees Celsius. This high temperature is crucial for effectively killing off any microorganisms present.

4. **Duration of Sterilization**:
- The tissue culture medium should be maintained at this temperature for a specific duration, typically around 15 minutes, to ensure complete sterilization.

5. **Conclusion**:
- Therefore, the correct answer to the question is that the sterilization of tissue culture medium is done using an autoclave at 120 degrees Celsius for 15 minutes.

### Final Answer:
The sterilization of tissue culture medium is done by autoclaving at 120 degrees Celsius for 15 minutes.

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