It shows correct chronological order of the events occurring during callus culture

Options

A. callus - cel division explant - cytokinin - acquire meristematic activity
B. explant - callus -cell division - cytokinins - cells acquire meristematic property
C. explant -cell divisin -callus -cytokinins -cells acquire meristematic property
D. callus -= explant -cell division -cytokinin -cell acquire meristematic property

Answer

### Step-by-Step Solution:

1. **Understanding Callus Culture**: Callus culture is a part of tissue culture where plant tissues are grown in vitro (outside the organism) under controlled conditions.

2. **Starting with Explant**: The process begins with the selection of an explant, which is a small piece of plant tissue taken from a donor plant.

3. **Cell Division**: Once the explant is placed in a suitable culture medium, it undergoes cell division. This is a crucial step as it leads to the proliferation of cells.

4. **Formation of Callus**: As a result of continuous cell division, a mass of undifferentiated cells, known as callus, is formed.

5. **Adding Hormones**: To enhance growth and induce differentiation, hormones such as cytokinins are added to the culture medium. These hormones stimulate the cells to divide and develop further.

6. **Acquisition of Meristematic Properties**: Finally, the cells in the callus can acquire meristematic properties, which means they can differentiate into various types of plant tissues and eventually develop into a whole plant.

7. **Correct Chronological Order**: Therefore, the correct chronological order of events occurring during callus culture is:
- Explant → Cell Division → Callus Formation → Addition of Cytokinins → Acquisition of Meristematic Properties.