Select the option which is not correct with respect to enzyme action

Options

A. Malonate is a competitive inhibtior of succines dehydrogenase
B. Substrate binds with enzyme at its active site
C. Addition of lot of succinate does not regenase by malonate
D. A non-competitve inhibitor binds the enzyme at a site distinct form that which binds the substrate.

Explanation

To solve the question regarding which option is not correct with respect to enzyme action, we will analyze each statement provided.

### Step-by-Step Solution:

1. **Understanding Enzyme Action**: Enzymes are biological catalysts that speed up chemical reactions in the body. They have specific sites where substrates bind, known as active sites.

2. **Analyzing the Statements**:
- **Statement 1**: "Malonate is a competitive inhibitor of succinate dehydrogenase."
- **Analysis**: This statement is correct. Malonate competes with succinate for the active site of succinate dehydrogenase, thus inhibiting the enzyme's action.

- **Statement 2**: "Substrate binds with enzyme at its active site."
- **Analysis**: This statement is also correct. Substrates indeed bind to the active site of enzymes to undergo a reaction.

- **Statement 3**: "Addition of a lot of succinate does not regenerate by malonate."
- **Analysis**: This statement is incorrect. In the presence of a competitive inhibitor like malonate, increasing the concentration of succinate can overcome the inhibition, thus allowing the reaction to proceed. Therefore, this statement is false.

- **Statement 4**: "A non-competitive inhibitor binds the enzyme at a site distinct from that which binds the substrate."
- **Analysis**: This statement is correct. Non-competitive inhibitors bind to an allosteric site (not the active site), which can inhibit the enzyme regardless of whether the substrate is bound.

3. **Identifying the Incorrect Statement**: Based on the analysis, the incorrect statement is the third one: "Addition of a lot of succinate does not regenerate by malonate."

### Final Answer:
The option which is not correct with respect to enzyme action is: **"Addition of a lot of succinate does not regenerate by malonate."**

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