Thylakoids removed from chloroplasts were kept in illuminated culture having `CO_2` and water. It did not produce sugar because of

Options

A. absence of enzymes
B. non linking of PS I and PS II
C. absence of light trapping molecules
D. non formation of assimilatory power

Answer

To answer the question, we need to analyze the role of thylakoids in the process of photosynthesis and why their removal would prevent sugar production, even when CO2 and water are present.

### Step-by-Step Solution:

1. **Understanding Thylakoids**: Thylakoids are membrane-bound structures within chloroplasts where the light-dependent reactions of photosynthesis occur. They contain chlorophyll and other pigments that capture light energy.

2. **Function of Thylakoids**: The light-dependent reactions convert light energy into chemical energy in the form of ATP and NADPH. These molecules are essential for the subsequent light-independent reactions (Calvin cycle), where CO2 is fixed into sugars.

3. **Removal of Thylakoids**: When thylakoids are removed from chloroplasts, they lose their structural and functional integrity. They cannot perform the light-dependent reactions effectively without being in the chloroplast environment.

4. **Illuminated Culture with CO2 and Water**: Even though the culture is illuminated and contains CO2 and water, the absence of thylakoids means that the necessary light-dependent reactions cannot occur. This leads to a lack of ATP and NADPH production.

5. **Absence of Enzymes**: The thylakoids also contain specific enzymes required for the light-dependent reactions. Without these enzymes, the process of capturing light energy and converting it into chemical energy is hindered.

6. **Conclusion**: Since ATP and NADPH are not produced, the Calvin cycle cannot proceed to fix CO2 into sugars. Therefore, the absence of enzymes and the inability to perform the light-dependent reactions result in no sugar production.

### Final Answer:
The thylakoids did not produce sugar because of the **absence of enzymes** necessary for the light-dependent reactions.

---