Which one of the following sub processes does not take place during protein synthesis in eukaryotes?

Options

A. Translocation of m-RNA relative to t-RNA
B. Activation of ribosomes
C. Methionine deformylation
D. Termination

Answer

To answer the question "Which one of the following sub-processes does not take place during protein synthesis in eukaryotes?", we will analyze the options provided and understand the processes involved in protein synthesis.

### Step-by-Step Solution:

1. **Understanding Protein Synthesis**:
- Protein synthesis in eukaryotes occurs in two main stages: transcription (in the nucleus) and translation (in the cytoplasm). The translation process involves ribosomes, mRNA, tRNA, and amino acids.

2. **Identifying the Options**:
- The options given are:
- A) Translocation of mRNA relative to tRNA
- B) Activation of ribosomes
- C) Methionine deformylation
- D) Termination

3. **Analyzing Each Option**:
- **A) Translocation of mRNA relative to tRNA**: This is a crucial step in translation where the ribosome moves along the mRNA, allowing the tRNA to bring the correct amino acids. This process occurs in eukaryotes.
- **B) Activation of ribosomes**: This refers to the assembly of ribosomal subunits and initiation factors to begin translation. This is also a necessary step in eukaryotic protein synthesis.
- **C) Methionine deformylation**: In prokaryotes, the initial amino acid is N-formylmethionine (fMet), which undergoes deformylation. However, in eukaryotes, the initial amino acid is methionine, and deformylation does not occur.
- **D) Termination**: This is the final step in translation where the completed polypeptide chain is released from the ribosome. This process is present in eukaryotes.

4. **Conclusion**:
- The only option that does not take place during protein synthesis in eukaryotes is **C) Methionine deformylation**.

### Final Answer:
C) Methionine deformylation