Given below are the steps of DNA fingerprinting in random sequence:-
(i) Isolation of DNA.
(ii) Separation of DNA fragements by electrophoresis,
(iii) Hybridisation using labelled VNTR probe. ltBrgt (iv). Transferring of separeted DNA fragments to synthetic membranes, such as nitrocellulose or nylon.
(v) digestion of DNA by restriction endonucleases,
(vi). Detection of hybridised DNA fragments by auto radio-graphy.
which of the following is the correct sequence:-

Options

A. (i),(v),(ii),(iv),(iii),(vi)
B. (i),(ii),(v),(iv),(iii),(vi)
C. (i),(ii),(iv),(iii),(iv),(vi)
D. (i),(ii),(iii).(v),(iv),(vi)

Answer

To arrange the steps of DNA fingerprinting in the correct sequence, we will follow the logical order of the processes involved. Here is the step-by-step solution:

### Step-by-Step Solution:

1. **Isolation of DNA (Step i)**: The first step in DNA fingerprinting is to isolate the DNA from the sample. This is crucial as we need pure DNA to work with for further analysis.

2. **Digestion of DNA by Restriction Endonucleases (Step v)**: After isolating the DNA, the next step is to digest the DNA using restriction endonucleases. These enzymes cut the DNA at specific sequences, resulting in fragments of varying lengths.

3. **Separation of DNA Fragments by Electrophoresis (Step ii)**: Once the DNA is digested into fragments, the next step is to separate these fragments using electrophoresis. This technique allows us to separate DNA fragments based on their size.

4. **Transferring of Separated DNA Fragments to Synthetic Membranes (Step iv)**: After separation, the DNA fragments are transferred to a membrane, such as nitrocellulose or nylon. This step is important for immobilizing the fragments for further analysis.

5. **Hybridization Using Labeled VNTR Probe (Step iii)**: In this step, a labeled probe that binds to specific variable number tandem repeats (VNTRs) in the DNA is introduced. This probe is essential for identifying specific DNA sequences.

6. **Detection of Hybridized DNA Fragments by Autoradiography (Step vi)**: Finally, the last step involves detecting the hybridized DNA fragments using autoradiography. This technique allows visualization of the DNA fragments that have bound to the labeled probe.

### Correct Sequence:
The correct sequence of steps for DNA fingerprinting is:
1. Isolation of DNA (i)
2. Digestion of DNA by restriction endonucleases (v)
3. Separation of DNA fragments by electrophoresis (ii)
4. Transferring of separated DNA fragments to synthetic membranes (iv)
5. Hybridization using labeled VNTR probe (iii)
6. Detection of hybridized DNA fragments by autoradiography (vi)

### Final Answer:
The correct sequence is: **i, v, ii, iv, iii, vi**.