While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine=29 %, Guanine= 17%, Cytosine=32%, Thymine=17 %, Considering the Chargaff's rule it can be concluded that

Options

A. it is a double stranded circular DNA
B. it is single stranded DNA
C. it is a double stranded linear DNA
D. no conclusion can be drawn

Answer

To solve the question regarding the analysis of DNA and the application of Chargaff's rule, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Chargaff's Rule**:
Chargaff's rule states that in double-stranded DNA, the amount of adenine (A) is equal to thymine (T), and the amount of guanine (G) is equal to cytosine (C). Additionally, the total amount of purines (A + G) should equal the total amount of pyrimidines (C + T).

2. **List the Given Data**:
- Total nucleotides = 5386
- Adenine (A) = 29%
- Guanine (G) = 17%
- Cytosine (C) = 32%
- Thymine (T) = 17%

3. **Calculate the Percentages**:
- Calculate the actual numbers of each nucleotide based on the total:
- A = 29% of 5386 = 0.29 * 5386 ≈ 1562
- G = 17% of 5386 = 0.17 * 5386 ≈ 915
- C = 32% of 5386 = 0.32 * 5386 ≈ 1723
- T = 17% of 5386 = 0.17 * 5386 ≈ 915

4. **Check Chargaff's Pairing**:
- According to Chargaff's rule:
- A should equal T: 1562 (A) ≠ 915 (T) → Not equal
- G should equal C: 915 (G) ≠ 1723 (C) → Not equal

5. **Check the Purine and Pyrimidine Rule**:
- Total purines (A + G) = 1562 + 915 = 2477
- Total pyrimidines (C + T) = 1723 + 915 = 2638
- Purines ≠ Pyrimidines: 2477 ≠ 2638

6. **Conclusion**:
Since neither of the conditions of Chargaff's rule is satisfied, we can conclude that this DNA is not double-stranded. Therefore, it must be single-stranded DNA.

### Final Answer:
The conclusion is that the DNA is **single-stranded DNA**.