If the sequence of nitrogen bases of the coding strand of DNA in a transcription unit is `5'-A T G A A T G -3'`, the sequence of bases in its RNA transcript would be

Options

A. 5' -A U G A A U G-3'
B. 5' - U A C U U A C - 3'
C. 5' - C A U U C A U - 3'
D. 5' - G U A A G U A- 3'

Answer

To determine the sequence of the RNA transcript from the given coding strand of DNA, we will follow these steps:

### Step 1: Identify the Coding Strand
The coding strand of DNA is given as:
5'-A T G A A T G-3'

### Step 2: Understand Base Pairing Rules
In RNA, the base pairing rules differ slightly from DNA:
- Adenine (A) pairs with Uracil (U) instead of Thymine (T).
- Thymine (T) in DNA pairs with Adenine (A) in RNA.
- Cytosine (C) pairs with Guanine (G) and vice versa.

### Step 3: Determine the Template Strand
The template strand is complementary to the coding strand and runs in the opposite direction. Therefore, we need to find the complementary bases for the coding strand:
- The complementary bases for the coding strand (5'-A T G A A T G-3') will be:
3'-T A C T T A C-5'

### Step 4: Transcribe the RNA Sequence
Now, we will transcribe the RNA sequence from the template strand. The RNA sequence is synthesized in the 5' to 3' direction, using the template strand:
- From the template strand:
- T pairs with A
- A pairs with U
- C pairs with G
- T pairs with A
- T pairs with A
- A pairs with U
- C pairs with G

Thus, the RNA sequence will be:
5'-A U G A A U G-3'

### Final Answer
The sequence of bases in the RNA transcript is:
**5'-A U G A A U G-3'**