To isolate protoplast, one needs

Options

A. pectinase
B. cellulase
C. both pectinase and cellulase
D. chitinase

Answer

To isolate protoplast, one needs to follow these steps:

1. **Understanding Protoplast**: Protoplasts are plant cells that have had their cell walls removed. This means that to isolate protoplasts, we must first understand what they are and their significance in plant biology.

2. **Identifying the Requirement**: The isolation of protoplasts is crucial for various biotechnological applications, including somatic hybridization. This process requires the breakdown of the cell wall to release the protoplast.

3. **Using Digestive Enzymes**: To isolate protoplasts effectively, specific digestive enzymes are needed. The two primary enzymes used for this purpose are:
- **Pectinase**: This enzyme breaks down pectin, a component of the plant cell wall.
- **Cellulase**: This enzyme breaks down cellulose, another major component of the cell wall.

4. **Excluding Unnecessary Enzymes**: While other enzymes like chitinase exist, they are not required for protoplast isolation since chitinase is involved in the digestion of chitin, which is found in fungal cell walls, not plant cell walls.

5. **Conclusion**: Therefore, to isolate protoplasts, one needs both pectinase and cellulase.

**Final Answer**: To isolate protoplast, one needs digestive enzymes such as pectinase and cellulase.

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