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An antibiotic resistance gene in a vecto...

An antibiotic resistance gene in a vector usually helps in the selection of :

A

competent cells

B

transformed cells

C

recombinant cells

D

none of the above

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To solve the question regarding the role of an antibiotic resistance gene in a vector, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding the Context**: - The question asks about the function of an antibiotic resistance gene in a vector. Vectors are DNA molecules used to deliver genetic material into a host cell. 2. **Identifying the Components**: - Bacterial cells typically contain chromosomal DNA and may also have plasmids, which are small, circular DNA molecules separate from chromosomal DNA. Plasmids can carry genes, including those that confer antibiotic resistance. 3. **Role of Antibiotic Resistance Genes**: - When a plasmid containing an antibiotic resistance gene is introduced into a bacterial cell (a process known as transformation), the bacteria that successfully take up this plasmid will gain the ability to resist specific antibiotics. 4. **Selection of Transformed Cells**: - In a laboratory setting, after the transformation process, the bacteria are exposed to an antibiotic. Only those bacteria that have taken up the plasmid (and thus express the antibiotic resistance gene) will survive in the presence of the antibiotic. This allows researchers to distinguish between transformed cells (those that have taken up the plasmid) and non-transformed cells (those that have not). 5. **Conclusion**: - Therefore, the antibiotic resistance gene in a vector is crucial for the selection of transformed cells, as it enables the identification of which cells have successfully incorporated the plasmid. ### Final Answer: An antibiotic resistance gene in a vector usually helps in the selection of **transform cells**. ---
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