Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts ?

Options

A. Laboratory flask of largest capacity
B. A stirred tank bioreactor without inlets and outlets
C. A continuous culture system
D. Any of the above

Answer

To determine the best option for producing a recombinant protein in large amounts, let's analyze the given choices step by step.

### Step 1: Understand the options
The options provided are:
1. Laboratory flask of largest capacity
2. A state tank bioreactor without inlets and outlets
3. A continuous culture system
4. Any of the above

### Step 2: Evaluate each option
- **Laboratory flask of largest capacity**: While a larger flask can hold more culture, it does not provide the necessary conditions for optimal growth and protein production over time.

- **A state tank bioreactor without inlets and outlets**: This setup would not allow for the exchange of nutrients or removal of waste products, which are critical for sustaining cell growth and maximizing protein yield.

- **A continuous culture system**: This system allows for the continuous addition of nutrients and the removal of waste products. It maintains optimal growth conditions and can lead to higher yields of recombinant proteins.

- **Any of the above**: This option suggests that any of the previous methods could be equally effective, which is not the case based on the evaluations.

### Step 3: Conclusion
Based on the analysis, the best choice for producing a recombinant protein in large amounts is the **continuous culture system**. This system is specifically designed to optimize growth conditions and maximize yield.

### Final Answer
The correct option is **a continuous culture system**.

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