The technique for breakage of DNA fragment and inserting it into another DNA molecule, is related to

Options

A. gene splicing
B. gene cloning
C. gene typing
D. DNA finger printing

Answer

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks about the technique that involves breaking a DNA fragment and inserting it into another DNA molecule. This is a fundamental process in biotechnology.

2. **Identifying the DNA Fragment**: In the context of biotechnology, the DNA fragment typically refers to a specific gene of interest that we want to manipulate or study.

3. **Using a Plasmid**: A common method for inserting a DNA fragment is to use a plasmid, which is a small, circular piece of DNA found in bacteria. Plasmids can replicate independently of chromosomal DNA.

4. **Cutting the DNA**: To insert the gene of interest into the plasmid, both the plasmid and the DNA fragment must be cut. This is usually done using restriction enzymes, which create specific cuts in the DNA at known sequences.

5. **Inserting the Gene**: After cutting the plasmid, the gene of interest is inserted into the open plasmid. This can be facilitated by using ligase enzymes that seal the DNA fragments together, creating a recombinant DNA molecule.

6. **Cloning the Plasmid**: Once the plasmid contains the gene of interest, it can be introduced into a host cell (often a bacterial cell). The host cell will then replicate the plasmid along with its own DNA, effectively cloning the gene of interest.

7. **Conclusion**: The entire process described is known as **gene cloning**, which is the technique for breaking a DNA fragment and inserting it into another DNA molecule.

### Final Answer:
The technique for breakage of DNA fragment and inserting it into another DNA molecule is related to **gene cloning**.