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Ligase helps in...

Ligase helps in

A

removal of few genes

B

translation

C

inserting few genes in DNA

D

bringing transversion in chromosomes

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The correct Answer is:
**Step-by-Step Solution:** 1. **Understanding Ligase**: Ligase is an enzyme that plays a crucial role in molecular biology. It is often referred to as a "joining enzyme" because of its function in connecting DNA fragments. 2. **Function of Ligase**: The primary function of ligase is to seal gaps between DNA fragments. It acts like a molecular glue that helps to join these fragments together. 3. **Formation of Phosphodiester Bonds**: Ligase facilitates the formation of phosphodiester bonds between the nucleotides of the DNA fragments. This is essential for creating a continuous DNA strand. 4. **Analyzing the Options**: - **Option A (Removal of fusions)**: This is incorrect because ligase does not remove genes; it helps in joining them. - **Option B (Translation)**: This is also incorrect as translation refers to the synthesis of proteins from mRNA, which is not a function of ligase. - **Option C (Inserting fusions in DNA)**: This option is correct. Ligase helps in inserting genes into DNA by forming phosphodiester bonds. - **Option D (Bringing transversion in chromosomes)**: This is incorrect as ligase does not induce transversions in chromosomes. 5. **Conclusion**: Based on the analysis of the options, the correct answer is **Option C**, which states that ligase helps in inserting fusions in DNA. ---
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A: DNA ligase plays an important role in recombinant DNA technology. R: The linking of antibiotic resistance gene with plasmid vector became possible by enzyme DNA ligase

Assertion: DNA ligase plays an important role in recombinant DNA technology. Reason: The linking of antibiotic resistance gene with plasmid vector became possible by enzyme DNA ligase

Explain the mechanism of DNA repication with the help of a replication fork. What role does the enzyme DNA-ligase play in a DNA replication fork ? OR Construct and label a transcription unit from which the RNA segment given below has been transcribed. Write the complete name of the enzyme that transcribed this RNA.

TRUEMEN BIOLOGY ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-MULTIPLE CHOICE QUESTIONS
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  2. The first successfully cloned mammal (animal) that gained worldwide pu...

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  3. Ligase helps in

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  4. Construction of a recombinant, DNA involves (A) cleaving of a recombin...

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  5. Who is regarded as 'father of Genetic engineering'? (A) Cohen (B) Boy...

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  6. The term biotechnology was given in 1919 by (A) Arber (B) Nathans (C)...

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  7. Cutting of DNA at specific location by restriction enzymes is populary...

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  8. How many types of restriction endonucleases are present ?

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  9. which type of restriction enzymes are used in recombinant DNA technolo...

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  10. The first restriction endonuclease was:

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  11. Who is the father of biotechnology in India ? (A) Prof. V.L. Chopra (...

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  12. Taq polymerase enzyme is used in

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  13. Which of the following is a method of gene transfer ?

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  14. 'Lal Bahadur Shastri centre for Advanced Research in Biotechnology'' i...

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  15. A gene carried by recombinant DNA is cloned when

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  16. In recombinant DNA technology, a plasmid vector is cleaved by

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  17. Becoming an expert on gel electrophoresis, you are asked to examine a ...

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  18. Plasmids are used in genetic engineering because they are

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  19. Cloning is means to

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  20. The palindromes are groups of letters that form the …….when read in bo...

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