Which of the following recent techniques is used for separating fragments of DNA ?

Options

A. northern blotting
B. southern blotting
C. eastern blotting
D. western blotting

Answer

To answer the question about which recent technique is used for separating fragments of DNA, we can follow these steps:

### Step-by-Step Solution:

1. **Identify the Techniques**: The options given are northern blotting, southern blotting, eastern blotting, and western blotting. It's important to understand what each of these techniques is used for.

2. **Understand the Purpose of Each Technique**:
- **Northern Blotting**: This technique is used for the detection of specific RNA sequences.
- **Southern Blotting**: This technique is specifically designed for the detection and separation of DNA fragments.
- **Eastern Blotting**: This is a less common technique used for the analysis of proteins, focusing on post-translational modifications.
- **Western Blotting**: This technique is used for the detection of specific proteins in a sample.

3. **Focus on DNA Fragment Separation**: Since the question specifically asks for a technique that separates DNA fragments, we can eliminate northern, eastern, and western blotting, as they do not pertain to DNA.

4. **Conclude with the Correct Technique**: The technique that is used for separating fragments of DNA is **Southern Blotting**. This method involves the transfer of DNA fragments from a gel to a solid support, such as a nitrocellulose filter, and is commonly used in DNA fingerprinting and forensic studies.

### Final Answer:
The correct technique used for separating fragments of DNA is **Southern Blotting**.

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