The enzymes used for separating the cells for protoplast culture are

Options

A. cellulase and pectinase
B. protease and lipase
C. gyrase and helicase
D. endonuclease and ligase

Answer

To answer the question regarding the enzymes used for separating cells for protoplast culture, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Protoplasts**: Protoplasts are plant cells that have had their cell walls removed. This is an essential step in protoplast culture, which allows for various biotechnological applications.

2. **Identifying the Enzymes**: The primary enzyme used to remove the cell wall is **cellulase**. Cellulase breaks down cellulose, which is a major component of the plant cell wall.

3. **Role of Pectinase**: In addition to cellulase, **pectinase** is also used. Pectinase helps to break down pectin, which is another component that holds plant cells together. This is important for separating individual cells after the cell wall is removed.

4. **Eliminating Incorrect Options**:
- **Proteases** are enzymes that digest proteins, not cell walls.
- **Lipases** digest lipids and are not involved in cell wall removal.
- **Gyrase and helicase** are related to DNA replication and do not play a role in protoplast culture.
- **Endonucleases** and **ligases** are involved in DNA manipulation, not in the separation of cells.

5. **Conclusion**: The correct enzymes for separating cells for protoplast culture are **cellulase** and **pectinase**. Therefore, the answer to the question is that the enzymes used for separating the cells for protoplast culture are primarily **cellulase** and **pectinase**.