Which one of the following palindromic base sequences in DNA can be easily cut a about the middle by some particular restriction enzyme ?

Options

A. 5' ___________ CGTTCG ____________3'
3' ________________ ATGGTA ____________ 5'
B. 5' ___________ GATATG _____________ 3'
3' __________ CTACTA ___________ 5'
C. 5' ___________ GAATTCC ___________ 3'
3' _____________CTTAAG _____________ 5'
D. 5' ____________ CACGTA _____________ 3'
3' ___________ CTCAGT __________ 5'

Answer

To solve the question regarding which palindromic base sequence in DNA can be easily cut about the middle by a particular restriction enzyme, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Palindromic Sequences**:
- A palindromic sequence in DNA is one that reads the same forwards and backwards on complementary strands. For example, if one strand reads 5'-GAATTC-3', the complementary strand reads 3'-CTTAAG-5'.

2. **Identifying Restriction Enzymes**:
- Restriction enzymes, also known as restriction endonucleases, recognize specific palindromic sequences in DNA and cut the DNA at or near these sites. Each restriction enzyme has a unique recognition sequence.

3. **Recognizing the Sequence**:
- The question provides options for palindromic sequences. We need to identify which of these sequences is specifically recognized by a known restriction enzyme.

4. **Example of a Common Restriction Enzyme**:
- One of the well-known restriction enzymes is EcoRI, which recognizes the palindromic sequence GAATTC. This sequence is cut between the G and the A, resulting in sticky ends.

5. **Conclusion**:
- Based on the information provided and the understanding of restriction enzymes, the palindromic base sequence that can be easily cut about the middle by EcoRI is **GAATTC**.

### Final Answer:
The palindromic base sequence in DNA that can be easily cut about the middle by a particular restriction enzyme is **GAATTC**.