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The vector for T-DNA is :...

The vector for T-DNA is :

A

Thermus aquaticus

B

Salmonella typhimurium

C

Agrobacterium tumefaciens

D

Escherichia coli

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The correct Answer is:
C
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Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created. (a) How are the desirable DNA sequences cut ? (b) Explain the technique used to separate the cut fragments. (c) How are the resultant fragments joined to the vector DNA molecule ?

The main cloning vectors used for DNA sequencing in the Human Genome Project are _________.

Draw the vector DNA segment and foreign DNA segments after the action of EcoRI and label the sticky and produced.

In recombinant DNA technique, the term vector refers to :

In recombinant DNA technique, the term vector refers to :

The vector used in the first successful recombinant DNA was from :

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TRUEMEN BIOLOGY ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-MULTIPLE CHOICE QUESTIONS
  1. In genetic engineering, a DNA segment (gene) of interest, is translate...

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  2. DNA or RNA segment tagged with a radioactive molecule is called

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  3. The vector for T-DNA is :

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  4. Which of the following is a plasmid ?

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  5. There is a restriction endonuclease called Eco RI. What does 'co' part...

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  6. Which of the following is correctly matched?

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  7. PCR proceeds in three distinct steps governed by temperature they are ...

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  8. Restriction enzymes are used to cut

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  9. Which one of the following technique made it possible to genetically e...

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  10. Given below is a sample of a portion of DNA strand giving the base seq...

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  11. Agarose extracted from sea weeds finds use in

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  12. cDNA is

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  13. PCR and restriction Fragments length Polymorphism are the methods for

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  14. The restriction enzyme(s) used in recombinant DNA technology that make...

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  15. Which one is a true statement regarding DNA polymerase used in PCR

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  16. The usual source of restriction endonucleases used in gene clonning is

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  17. Restriction enzyme Eco RI cuts the DNA between bases G and A only when...

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  18. Biolistics (gene-gun) is suitable for

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  19. DNA parts which can switch their position are

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  20. In a genetic engineering experiment restriction enzymes can be used fo...

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