The restriction enzyme(s) used in recombinant DNA technology that makes staggered cuts in DNA leaving sticky ends is/are

Options

A. Eco R I
B. Hind III
C. Bam H I
D. all of these

Answer

**Step-by-Step Solution:**

1. **Understanding Restriction Enzymes**:
- Restriction enzymes are proteins that cut DNA at specific sequences. They are crucial in recombinant DNA technology.
- They can create two types of ends after cutting: sticky ends and blunt ends.

2. **Defining Sticky Ends**:
- Sticky ends are created when a restriction enzyme makes a staggered cut in the DNA, resulting in short single-stranded overhangs. These overhangs can easily anneal with complementary DNA strands.

3. **Identifying the Options**:
- The question provides three options: EcoR1, HIN3, and BamH1. We need to determine which of these enzymes produces sticky ends.

4. **Analyzing EcoR1**:
- The recognition sequence for EcoR1 is 5' GAA TTC 3'.
- It cuts between the G and A on both strands, resulting in overhangs of AATT, which are sticky ends.

5. **Analyzing HIN3**:
- The recognition sequence for HIN3 is 5' AAG CTT 3'.
- It cuts between the A and A on both strands, resulting in overhangs of AGCT, which are also sticky ends.

6. **Analyzing BamH1**:
- The recognition sequence for BamH1 is 5' GGA TCC 3'.
- It cuts between the G and A on both strands, resulting in overhangs of GATCC, which are sticky ends as well.

7. **Conclusion**:
- All three enzymes (EcoR1, HIN3, and BamH1) produce sticky ends. Therefore, the correct answer is option D: all of these.

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