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Restriction enzyme Eco RI cuts the DNA b...

Restriction enzyme Eco RI cuts the DNA between bases G and A only when the sequence in DNA is

A

GATATC

B

GAATTC

C

GATTCC

D

GAACTT

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To determine the DNA sequence that the restriction enzyme Eco RI cuts, we can follow these steps: ### Step-by-Step Solution: 1. **Understanding Restriction Enzymes**: - Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences. Each restriction enzyme recognizes a particular sequence of nucleotides. 2. **Identifying Eco RI**: - Eco RI is a specific restriction enzyme that is derived from the bacterium E. coli. The strain of E. coli from which Eco RI is isolated is called RY113. 3. **Recognizing the Cut Site**: - Eco RI recognizes and cuts the DNA at a specific sequence of six base pairs. The sequence that Eco RI targets is crucial for understanding where the cuts will occur. 4. **Determining the Sequence**: - The specific sequence that Eco RI recognizes is G A A T T C. This means that Eco RI will cut the DNA between the bases G and A in this sequence. 5. **Understanding Palindromic Sequences**: - The sequence G A A T T C is a palindromic sequence. This means that it reads the same forwards and backwards. In the context of DNA, this is important because it allows the enzyme to bind and cut effectively. 6. **Final Answer**: - Therefore, the DNA sequence that Eco RI cuts is G A A T T C.
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TRUEMEN BIOLOGY ENGLISH-BIOTECHNOLOGY : PRINCIPLES AND PROCESSES-MULTIPLE CHOICE QUESTIONS
  1. Which one is a true statement regarding DNA polymerase used in PCR

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  2. The usual source of restriction endonucleases used in gene clonning is

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  3. Restriction enzyme Eco RI cuts the DNA between bases G and A only when...

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  4. Biolistics (gene-gun) is suitable for

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  5. DNA parts which can switch their position are

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  6. In a genetic engineering experiment restriction enzymes can be used fo...

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  7. The given figure is the diagrammatic representation of the E. coli vec...

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  8. Which one of the following is a case of wrong matching?

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  9. DNA polymerase can be obtained from

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  10. Viruses were first Crystallised by

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  11. The colonies of recombinant bacteria appear white in contrast to blue ...

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  12. DNA fragments generated by the restriction endonucleases in a chemical...

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  13. Which of the following is not correctly matched for the organism and i...

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  14. Which vector can clone only a small fragment of DNA ?

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  15. The cutting of DNA at specific locations became possible with the disc...

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  16. The introduction of t-DNA into plants involves tumefaciens

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  17. The DNA molecule to which the gene of interest is integrated for cloni...

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  18. The taq polymerase enzyme is obtained from

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  19. Which of the following is a restriction endonuclease

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  20. Stirred-tank bioreactors have been designed for :

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