Biolistics (gene-gun) is suitable for

Options

A. disarming pathogen vectors
B. transformation of plant cells
C. constructing recombinant DNA by joining with vectors
D. DNA fingerprinting

Answer

**Step-by-Step Solution:**

1. **Understanding Biolistics (Gene Gun):**
- Biolistics, also known as the gene gun, is a method used to introduce foreign DNA into cells. It involves the use of high-velocity microprojectiles that are coated with DNA.

2. **Identifying the Purpose of Biolistics:**
- The primary purpose of biolistics is to facilitate the transformation of plant cells. This is achieved by bombarding the cells with microprojectiles made of gold or tungsten that carry the DNA.

3. **Evaluating the Options:**
- The question asks for the suitability of biolistics in various processes. The options include:
- Disarming pathogen vectors (e.g., Agrobacterium tumefaciens)
- Transformation of plant cells
- Constructing recombinant DNA by joining with vectors
- DNA fingerprinting

4. **Analyzing Each Option:**
- **Disarming Pathogen Vectors:** Biolistics is not suitable for this process. Disarming involves cutting out specific DNA segments (like tDNA from Agrobacterium), which does not require the gene gun.
- **Transformation of Plant Cells:** This is the correct option. Biolistics is specifically designed to introduce DNA into plant cells, making them competent for transformation.
- **Constructing Recombinant DNA:** This process involves joining DNA fragments with vectors, which is not the primary function of biolistics.
- **DNA Fingerprinting:** This technique is unrelated to the biolistics method.

5. **Conclusion:**
- Based on the analysis, the most suitable process for biolistics (gene gun) is the transformation of plant cells.

**Final Answer:**
Biolistics (gene-gun) is suitable for the transformation of plant cells.

---