Which of the following restriction enzymes produces blunt ends

Options

A. (a) Sal l
B. (b) Eco RV
C. (c) Xho l
D. (d) Hind III

Answer

To determine which restriction enzyme produces blunt ends, we need to analyze the options provided and understand how different restriction enzymes function.

### Step-by-Step Solution:

1. **Understanding Restriction Enzymes**:
- Restriction enzymes, also known as molecular scissors, cut DNA at specific sequences known as restriction sites. They can produce two types of ends when they cut: blunt ends and sticky ends.

2. **Blunt Ends vs. Sticky Ends**:
- **Blunt Ends**: Produced when the enzyme cuts straight across both strands of the DNA at the same position, resulting in no overhangs.
- **Sticky Ends**: Produced when the enzyme cuts the DNA in a staggered manner, creating overhangs of 2-3 nucleotides.

3. **Analyzing Each Option**:
- **Option A: SalI**: This enzyme is known to produce sticky ends. Therefore, it is not the correct answer.
- **Option B: EcoRV**: This enzyme is known to produce blunt ends. It cuts the DNA straight across, making it the correct answer.
- **Option C: ZO1**: This enzyme produces sticky ends, so it is not the correct answer.
- **Option D: Hint3**: This enzyme also produces sticky ends, thus it is not the correct answer.

4. **Conclusion**:
- After analyzing all the options, the only restriction enzyme that produces blunt ends is **EcoRV**.

### Final Answer:
**EcoRV** is the restriction enzyme that produces blunt ends.