DNA fragments are :

Match the terms given in column I with their definitions in column II and select the correct answer from codes given below. {:(Column I,Column II),(A."Transformation",(i)"Sequences cut by restriction enzymes"),(B."Recognition site",(ii)"Process by which DNA fragments are separated based on their size"),(C."Gel electrophoresis",(iii)"Plasmid DNA that has incorporated human DNA"),(D."Recombinant DNA",(iv)"Process by which bacteria take up pieces of DNA from the environment"):}

Study the following statements regarding recombinant DNA technology and select the incorrect ones. (i) Taq polymerase extends the primers using the nucleotides provided in the reaction. (ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology. (iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis. (iv) Transformation is a procedure through which a piece of DNA is integrated in to the genome of a host bacterium. (v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture. (vi) Downstream processing is one of the steps of polymerase chain reaction.

DNA fragments can be separated and detected using_____.

Fill up the blank DNA fragment are joined b y ____________

The figure shows the restriction enzyme cutting sites (R1-R3) in wild type (n) and mutant (n) gene. If a radioactively labelled probe (that hybridises at a sequence close to R1) is used for detecting the presence of DNA fragments after gel electrophoresis and Southern blotting, which of the following band patterns will yout expect ? Note : L1 : wild type DNA, L2: mutant DNA

Okazaki fragments are :

DNA fragments move towards anode during Denaturation.