The chromatography does not depend upon

Options

A. Length of column
B. Solubility of compound
C. Physical state
D. Flow rate of the solvent

Answer

To answer the question regarding what chromatography does not depend upon, we can analyze the factors mentioned in the question: length of the column, solubility of the compound, physical state, and flow rate of the solvent.

### Step-by-Step Solution:

1. **Understanding Chromatography**:
Chromatography is a technique used for the separation and purification of mixtures. It involves a mobile phase (the solvent) and a stationary phase (the column or medium where separation occurs).

2. **Analyzing Each Factor**:
- **Length of the Column**:
The length of the column can affect the separation efficiency but is not a fundamental requirement for the chromatography process itself. Different lengths can provide different resolutions, but chromatography can still occur regardless of the column's length.

- **Solubility of the Compound**:
The solubility of the compounds being separated is crucial. If a compound is not soluble in the mobile phase, it cannot be transported through the column, making solubility a key factor in chromatography.

- **Physical State**:
The physical state (solid, liquid, gas) of the substances involved is also important. Chromatography typically requires the mobile phase to be in a liquid or gas state to facilitate the movement of compounds. Therefore, the physical state is significant.

- **Flow Rate of the Solvent**:
The flow rate of the solvent influences how quickly the compounds move through the column and can affect separation efficiency. Thus, it is a relevant factor in chromatography.

3. **Conclusion**:
Based on the analysis, the factor that chromatography does not depend upon is the **length of the column**. While it can influence the efficiency of separation, it is not a fundamental requirement for the process to occur.

### Final Answer:
Chromatography does not depend upon the **length of the column**.

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