The thermostable enzymes,'Taq' & ‘P&U’ i...

The thermostable enzymes,'Taq' & ‘P&U’ isolated form thermophilic becteria are _______

RNA Polymerases

DNA Polymerases

Restriction endonucleases

DNA ligases

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Enzyme that forms short primers are called__________

Give the name of the organism from where the thermostable DNA polymerase is isolated. State its role in genetic engineering.

In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction (PCR) ?

Bt toxin genes are isolated form a bacterium called _______ and incorporated into sereral crop plants.

Enzyme 'Taq polymerase' used in PCR, has been isolated from bacterium 1) Agrobacterium tumefaciens 2)Thermus aquaticus 3) Streptomyces albus 4) Escherichia coil

Which of the following statements does not hold true for restriction enzyme? (a) It recongnises a palindromic nucleotide sequence (b) It is an endonuclease. (c) It is isolated from viruses (d) It produces the same kind of sticky ends in different DNA molecules

Assertion : Use of chitinase enzyme is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason : Fungal cell wall is made up of fungal cellulose or chitin.

Which of the following statements are correct for the enzyme Taq polymerase ? (i) It remains active during the high temperature induced denaturation of dsDNA. (ii) It requires primers for carrying out the process of polymerisation. (iii) It synthesises the RNA region between the primers, using dNTPs and Mg^(2+) .

In a polymerase chain reaction after the denaturation step why the mixture needs to cool down to a lower temperature ? a) To permit specific annealing of the primers b) To give a halt to the reaction mixture c) To increase the activity of enzyme Taq polymerase d) To obtain the multiple copies of the DNA

USHA PUBLICATION-BIOTECHNOLOGY & ITS APPLICATIONS-EXERCISE

The process of separation & purification of expressed protein before m...
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The DNA fragments separated on an agarose gel can be visualised after ...
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The thermostable enzymes,'Taq' & ‘P&U’ isolated form thermophilic bect...
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Which of the following are used in gene cloning ?
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In genetic engineering, the terms vector is applied for :
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Who first invented PCR technique ?
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